FLIM, FCS and anisotropy measurements for structural biology and phase separation studies
- Abstract number
- 15
- Presentation Form
- Poster
- Corresponding Email
- [email protected]
- Session
- Poster Session
- Authors
- Maria Loidolt-Krueger (1), Marcelle Koenig (1), Fabio Barachati (1), Matthias Patting (1), Marcus Sackrow (1), Kamil Bobowski (1), Mathias Bayer (1), Evangelos Sisamakis (1), Felix Koberling (1), Rainer Erdmann (1)
- Affiliations
-
1. PicoQuant GmbH
- Abstract text
Quantitative single molecule and time-resolved fluorescence techniques offer new insights into many samples from various research areas such as dynamic structural biology or cellular mechanisms driven by phase separation.
Using biological condensates as an example, I will show how Fluorescence Lifetime Imaging (FLIM) and anisotropy imaging can observe dynamics of condensates and assess their heterogeneity.
In addition, single-molecule FRET (smFRET), fluorescence (cross-)correlation spectroscopy (F(C)CS) are complementary methods that can be combined to get a comprehensive picture of the distribution and dynamics of individual molecules inside and outside condensates.