Laser Light Engine Output Stability Characterization
- Abstract number
- 124
- Presentation Form
- Poster
- DOI
- 10.22443/rms.elmi2024.124
- Corresponding Email
- [email protected]
- Session
- Poster Session
- Authors
- Iain Johnson (1), Andrii Repula (1), Claudia Jaffe (1)
- Affiliations
-
1. Lumencor, Inc.
- Abstract text
Laser Light Engines consisting of as many as eight independently operable solid-state lasers merged
into a common optical train, provide a compact and operationally efficient illumination solution for
applications requiring rapid and reproducible switching between different output wavelengths. Shared
environmental and electronic control infrastructure is designed to produce consistent spatial and
temporal light output characteristics among the numerous elements of the laser array, wherein only
wavelengths remain as the lone variable. This presentation describes characterization of both continuous
and pulsed light output from the seven lasers of CELESTA Light Engines, over different timescales and
using a variety of photometric detectors. Temporal output stability over timescales from milliseconds to
days is critical for applications of the CELESTA Light Engine. For example, smFISH imaging protocols for
identification of thousands of transcripts across 1 cm2 tissue specimens may extend for more than 24
hours. Furthermore, these image acquisition protocols typically utilize sequential illumination from four or
more of the seven lasers. Therefore, it is essential that the temporal characteristics of the laser sources
are consistent.