Nanoscale mapping of the dynamics and organisation FGF/FGFR signalling initiation

Session

Poster Session

Authors

Yann Lanoiselée (1), Yong Li (3), David Fernig (3), Daniel Nieves (2)

Affiliations

1. Basque Center for Applied Mathematics
2. University of Birmingham
3. University of Liverpool

Keywords

Super-resolution, SMLM, SMT, PAINT, FGF, FGFR Signalling

Abstract text

Fibroblast Growth Factor (FGF) signalling plays pivotal roles in various cellular processes, ranging from development to tissue repair. Understanding the spatiotemporal dynamics and organization of FGF receptor (FGFR) signaling initiation at the nanoscale level is crucial for elucidating its regulatory mechanisms. Here, we employed single molecule localization microscopy (SMLM), single molecule tracking (SMT), and points accumulation for imaging in nanoscale topography (PAINT) imaging, to investigate the intricate details of FGF/FGFR signalling initiation.

Through SMLM, we were able to map the nanoscale organization of the FGFRs subfamilies and determine the change in their relative spatial distribution and clustering upon ligand stimulation. We use SMT and PAINT to track single FGF and FGFR, revealing their dynamic movements and interactions within the plasma membrane. This allowed us to probe how FGF distribution and confinement is governed in the glycocalyx of mammalian cells, and determine the rate of occupancy and binding kinetics of FGFRs by the ligand.

Our findings unveil the nanoscale dynamics and spatial organization of FGF/FGFR signalling initiation, shedding light on the mechanisms underlying how signal transduction is orchestrated in this receptor system.