Bruker
Monika Löschinger, Björn Eismann, Jürgen Mayer
Workshop Room 14
Light sheet microscopy is the most versatile imaging method, when imaging three dimensional fluorescent samples across all different biological scales. Diverse implementations of this technology have allowed researchers to visualize samples over time, ranging from sub cellular components up to multi-centimeter sized organisms in three dimensions.
In this workshop Luxendo presents two florescent microscopes and their respective applications at both ends of the scale. We present the TruLive3D Imager, a light sheet microscope, that is capable of imaging of living cells and organisms at sub-cellular resolution, over several days, without significantly affecting the viability nor the biology. In addition, the TruLive3D imager is showcased with our Photo-Manipulation (PM) Module, that uses a diffraction limited laser beam for photo-manipulating the sample.
Secondly, we present the Large Cleared Sample (LCS) light sheet microscope, capable of imaging a complete mouse in multiple colors at cellular resolution in its entry, within a short time frame.
What to expect: To overcome challenges of light sheet microcopy and the interaction with the sample, Luxendo has implemented a unified solution for all Luxendo microscope products in its
LuxBundle software. In this workshop we will focus on our seamless integration of a flexible and easy-to-use interface for smart image acquisition, fast 3D viewing of data, and powerful post-processing capabilities of the LuxBundle software. From simple linear workflows to complex multidimensional experiments, LuxBundle flexible design guides you seamlessly through the processes.
We will showcase exemplary light sheet imaging applications, starting from sample mounting to smart tiling experimental setup, selective ROI tracking, fast 3D imaging and smart data processing. We will highlight how the Luxendo software can be used for customized, smart microscopy workflows, supported by a fully scriptable open interface.
