Phasefocus
Peter Djali, Meetal Jotangia, Jessica Rickman
On Stand Workshop - Stand 38
‘Why is there no correlation between my in vitro and in vivo data?’ is a major question asked by cancer researchers. Investigating the mechanism of potential cancer therapies usually relies on the use of fluorescence markers and relatively high light levels which can ultimately perturb the natural function of cells. A solution is Livecyte; its label-free technique can reliably measure cell behaviour, identify mitotic cells and changes in the cell cycle without causing toxicity effects, thereby providing a more physiologically relevant assay model.
We’ll present how Livecyte’s single cell tracking and segmentation is perfect for investigating cancer candidate compounds. We’ll give an overview of how our analysis gives not only dynamic population information on the phenotypic changes characteristic of tumour behaviour but also delves down to the single cell level. We’ll show you how you can acquire multi-generational lineage data, magnifying changes in the cell cycle, particularly outlier cells which may show traces of drug resistance.
Through this workshop you can learn how Livecyte’s well designed dashboards tell you more about pharmacological effects on cancer cell division, invasion and metastasis by tracking your cancer cell behaviour such as proliferation, growth and motility.
With a deep dive using Livecytes explore results page you gain insight into cell cycle changes at a single cell level. Through changes in growth characteristics, such as individual cell dry mass, Livecyte is able to distinguish heterogeneity in cell division times in a seemingly homogenous population of cells. You can therefore distinguish how individual cells are responding to the drug and review a lineage tree comparing individual cells and their progeny over multiple generations.
