The ACQUIFER IM – Workflows for Smart Microscopy, High Content Screening and Automated Photomanipulation

15:40 – 16:40 BST, 5 June 2024 ‐ 1 hour

Room: Workshop Room 14

Bruker

Jochen Gehrig, Laurent Thomas

Workshop Room 14

The ACQUIFER IM is a flexible automated widefield fluorescence microscope designed for high-content imaging of cell cultures and small model organisms. Featuring a unique moving optical unit and stationary sample holder, it excels at imaging non-adherent and motion-sensitive specimens. Engineered for extended operation and extensive experiments, it offers innovative features and a user-friendly interface, requiring minimal maintenance.

The IM control software offers a user-friendly interface, allowing non-experts to configure imaging and screening protocols effortlessly. Advanced users can customize complex workflows using built-in scripting support. This open interface facilitates implementation of smart microscopy workflows. Additionally, the ACQUIFER Plate-Viewer software provides easy browsing and visualization of large high-content screening datasets, along with supervised feedback microscopy modules.

During this workshop, we will showcase the distinctive hardware design of the ACQUIFER IM and illustrate its applications in automated imaging. We will present our workflow for automated imaging, smart microscopy, and High Content Screening. Additionally, we will highlight applications of the optional laser module, which enables scaling up of challenging photomanipulation experiments such as photodamaging and fluorophore conversion.

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