elmi Community Room Workshop
Orestis Faklaris, Biocampus, CNRS, France & WG5 Group Members
Measure the Point Spread Function (PSF) of a microscope. The PSF is one of the key quality control elements of a microscope. Frequent measurements, done with the same tools, methods, protocols, and calculating robust metrics, can ensure the reproducibility of the scientific measurements done for biological experiments. During this workshop, we are going to show the participants how to mount a bead slide, how to perform an acquisition, and then how to analyze the PSF with open-source tools. We will give some troubleshooting for PSFs that have a strange shape or are far from the theoretically expected size. At this workshop, we are going to show the protocols and metrics that are defined in the framework of the QUAREP-LiMi consortium's WG5.