Phasefocus
Peter Djali, Meetal Jotangia, Jessica Rickman
On Stand Workshop - Stand 38
Cell therapy and regenerative medicine is fast becoming a critical area of research; cellular immunotherapies are already proving successful in the fight against cancer and there are a host of approved cellular regenerative therapies with >4000 ongoing clinical trials worldwide.
In vitro studies are key to developing effective cellular treatments, commonly using stems cells or progenitor cells as a starting point with various differentiation methods to incite specific lineages. Being able to investigate the specific subpopulation phenotypes without causing harm to cells is of utmost importance and equally important from early-stage discovery to clinic.
In this workshop you’ll learn how, through Livecyte’s Analyse software, you can isolate sub-populations within a heterogenous population based on both fluorescence and non-fluorescent based mechanisms. Navigate through Livecyte’s dashboards on a variety of cell characteristics ranging from morphology, motility to proliferation and growth to create and compare a full phenotypic profile of each sub-population and gain in-depth insights into differentiation of your cells.
Furthermore, we will show how to explore the function of your cells within an in vitro model of tissue repair with Livecyte’s wound healing assay platform. Become proficient in quantifying not only how quickly a scratch wound is closing, but why: for example, Livecyte’s single cell segmentation and tracking differentiates between cells migrating quicker or with more directionality. Its Wound Healing dashboard gives you a robust and reliable wound closure profile overcoming the limitations of classic scratch wound metrics and confluence-based approaches and ultimately adding an extra dimension when investigating collective migration, an inherent part of tissue repair and wound healing.
