Confocal.nl B.V
Janine Cravo, Dušan Popov-Čeleketić
Workshop Room 10
KEYWORDS: super resolution, confocal imaging, live cell imaging, deep imaging, REscan
One of the main goals of advanced optical microscopy is the development of systems enabling real-time live cell super resolution imaging. The most severe limitation towards this is the extreme phototoxicity of such systems. To tackle this and achieve cell-friendly super resolution imaging, Confocal.nl developed GAIA, the latest generation of the Point REscan confocal technology. This disruptive technology enables super resolution imaging in extremely cell friendly conditions. In addition, GAIA allows deep imaging beyond the diffraction limit while using ultra-low laser power.
GAIA is equipped with a switchable pinhole that allows enhanced flexibility, sensitivity, and optimal confocality. By Nyquisting every objective and enabling fast multicolour imaging over a large field of view (FOV), GAIA establishes itself as the most light-efficient super resolution confocal system on the market. GAIA is optimized for both VIS and NIR imaging as well as for super resolution with both high and low magnification objectives.
GAIA is an easy to use add-on to any widefield microscope. As it is uniquely capable to image beyond the diffraction limit using low magnification objectives and, consequently, to image live samples deeper than 500 µm while sustaining their viability over extended periods. As imaging up to 100 µm is considered deep in confocal and super resolution microscopy, we termed super resolution confocal imaging deeper than 500 µm as profound.