Carl Zeiss Microscopy
Ed Rea1, Chris Power2
1Carl Zeiss Ltd, United Kingdom. 2Carl Zeiss Microscopy GmbH, Germany
Workshop Room 11A
Understanding dynamic behaviour is vital to gaining insights into biological processes. Across all scales, dynamics provide a crucial insight, from movement of organisms, organs or cells, developmental changes in organisms or tissues to cell-cell interactions and intracellular molecular behaviour which one often cannot observe due to phototoxicity, slow acquisitions or too complicated software. ZEISS offers a wide range of systems & software to observe & analyse samples with minimal light exposure at high speed down to molecular resolution.
Fundamentals of imaging dynamic processes are of course matching the spatial and temporal resolution. In addition, it needs to be ensured that sample disturbance is kept to the absolute minimum - hence light exposure needs to be kept at bay by using the most sensitive method, regardless of whether whole organisms or single molecules are investigated.
ZEISS Dynamics Profiler uncovers molecular diffusion, concentration, and flow dynamics of fluorescent proteins in your living samples in a single, easy measurement. Delicate samples can be explored without excessive light exposure or prolonged experiment time.
Molecular dynamics experiments were often limited by lack of necessary equipment or the need for highly trained personnel. Dynamics Profiler can be easily added to a ZEISS confocal by utilizing the sensitive Airyscan detector. Now, any proficient confocal microscopy user can go beyond traditional confocal imaging to collect molecular dynamics information about a protein of interest. The wizard-guided workflow ensures precise acquisition settings and simple data quality control. Reference images aid in sample context and measurement position documentation. Comprehensible data visualization enables intuitive access to the information obtained. Adding molecular dynamics measurements to any of your current live sample experiments has never been easier.
Develop a more in-depth profile of the molecules in your current experiments, from cell cultures to organoids to whole organisms – even for bright and challenging samples. Examples of new dimensions uncovered by Dynamics Profiler include the transition of cellular condensates formed by liquid-liquid phase separation as measured by Asymmetric Diffusion. With Flow Analysis, measure speed and direction of fluorescent molecules moving in a bloodstream or in microfluidic systems, such as organ-on-a-chip experiments.
Raw data is saved with every measurement, enabling you to perform customized analyses, either immediately or when the scientific question arises later.
It’s easier than you think to add molecular dynamics measurements to your current confocal experiments.