Claudia Florindo, Aishwarya Sivakumar

Workshop Room 4B

The Dragonfly 600 is a high-speed confocal imaging platform that delivers matchless imaging capabilities across scales from nanometres to centimetres. The new features included are:

Andor's proprietary B-TIRF imaging modality, which delivers highly uniform TIRF imaging, is exceptionally easy to setup due to the optical feedback, and offers flexibility in its range to also image in HiLo;
A newly developed high-power laser engine (HLE) for super-resolution techniques and higher throughput imaging; and
The 3D super-resolution module combined with all imaging modalities (widefield, confocal and B-TIRF).

The Dragonfly spinning disk confocal is a complete multimodal system with outstanding versatility and exceptional performance in any scale, either Single-molecule imaging applications (SMLM), thick samples and live cell imaging.

In this workshop, we will present the Dragonfly 600, and the attendees will be able to see its key features live, such as:

SMLM paint imaging with Dragonfly 600 using DNA-PAINT samples.
B-TIRF imaging and its easy setup with the optical feedback
Imaging with confocal mode into deep thick samples while visualizing in 3D the imaging result.

Join us to understand how Dragonfly 600 can boost your research.

Figure legend: Dragonfly allows correlative/cross scale microscopy. a) image of 3 cell nuclei in which the nuclear pores were transiently labelled for NUP96 (nuclear pore protein). b) The same data set, zoomed a section in a) to show DNA-PAINT resolution of <20 nm, revealing the 8-fold symmetry of the nuclear pore complex c) DNA-origami 3x4 grid with ~20 nm Cy3B fluorophore separation, imaged with a similar B-TIRF protocol shows resolution <10 nm.

Dragonfly 600: From single molecule localization microscopy (SMLM) to large sample imaging