Fluorescence Imaging Optimisation with Virtex Real-Time Experiment Controller in Visiview 6.0 and Integration with Orbital Ring-TIRF Technology

17:10 – 18:10 BST, 5 June 2024 ‐ 1 hour

Photon Lines Ltd

Helmut Wurm1, Martyn Reynolds2

1Visitron GmBH, Germany. 2Photon Lines Ltd, United Kingdom

Workshop Room 18

This workshop will give an overview of the latest features in VisiView multidimensional imaging software and precise experiment timing control using the ViRTEx interface. Recent developments in Ring TIRF technology will also be presented.

The workflow oriented VisiView design offers an intuitive image acquisition platform for most complex multidimensional life science applications. The flexible and customisable hardware support of any microscope platform, scientific grade camera or high quality microscopy peripheral, including Confocal, FRAP or TIRF control offers each researcher the optimum solution.

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The ViRTEx-100/200 provides sophisticated experiment control, where precise timing is essential. Typically it is used in Confocal, FRAP and TIRF experiments where fast and highly accurate TTL synchronization of camera exposure periods is required with illumination devices like LED or laser systems. Furthermore for precise and stable Z-stack 3D image acquisition, synchronisation of Z-Focus Piezo movements is required at high speed, functionality fully supported by the ViRTEx-200 device.

The VisiTIRF-ORBITAL is a compact and powerful high speed 2D galvo driven spinning Ring-TIRF laser illumination system. It offers a large and evenly illuminated field of view to enable applications such as single molecule tracking or SMLM - Single Molecule Localisation Microscopy for superresolution imaging. Full 360 degree positioning of the laser spot by free circular diameter or elliptical trajectory at the back focal plane of the high aperture TIRF objective offers illumination with minimal fringes or shading gradients.