Super-resolution imaging has never been so easy

17:10 – 18:10 BST, 6 June 2024 ‐ 1 hour

Room: Workshop Room 13

Nikon Europe BV

Lisa Sanchez, Philipp Struntz

Workshop Room 13

Previously Nikon introduced the X-light V3 Spinning Disk coupled to DeepSIM System from CrestOptics. This year, understanding the increasing need for higher resolution imaging, we would like to introduce the stand-alone super-resolution modality DeepSIM. DeepSIM stand-alone is simple yet effective device delivering twice the resolution of widefield. This modality brings lattice SIM performance (light efficiency, high contrast, penetration depth) with an incredible ease of use. Incredibly compact, the DeepSIM is so far the only SIM device which can be coupled to an upright microscope.   

Advanced optical design and engineering solutions developed by both Nikon and CrestOptics meet very high-end specifications required by most facilities and users. 

• Enhanced resolution: down to 100 nm (XY) / 300 nm (Z) to observe finer details
• Do not be limited to thin samples with resolution improvement in depth (up to ~ 100 µm)
• Sample and experiment flexibility: 3 standard lattice patterns to match sample conditions (thickness, label density, dynamics) and compatible with low and high magnification objectives (from 20X up to 100X).
• User-friendly Nikon integration: easy calibration, reconstruction in one click, no complex settings, no expertise required
• Standard sample preparation: same as for confocal imaging 
• Compact module suitable for Nikon inverted and upright configurations
• Compatible with the same high NA objective line up as for confocal microscopy and works wonders with our Nikon silicon & water immersion, and get accurate 3D data
• Super-Resolution modality combined to Nikon’s exceptionally stable microscope set-up allows for: 
  • Wide variety of application ranging from live cell imaging, high-throughput imaging and depth imaging (up to ~ 100 µm)
  • Cover simple to complex imaging workflows thanks to Nikon’s Software platform NIS-Elements

Cleared mouse intestine section (0.55 mm thickness) with blood vessels in green and nuclei in red. Objective: 20X Air CFI Plan Apo Lambda, Nikon, 0.75 NA and 1mm WD.