Andy Hill¹, Martyn Reynolds¹, Lukas Krainer²

¹Photon Lines Ltd, United Kingdom. ²Prospective Instruments, Austria

Workshop Room 18

The experimental requirements for different imaging scenarios demand different sample mounting arrangements and flexible microscope setups are needed to meet this demand.

The MPX multimodal multiphoton microscope from Prospective Instruments with its two-photon integrated wavelength tunable fs laser and its built-in epi widefield fluorescence modality covers a wide range of different imaging needs. Its easy-to-use and flexible design provides for a large working space underneath the 360-frontend enabling microscopy from any angle, positioning and direction (upright or inverted). Multimodal imaging including two-photon (TPEF), higher harmonics (SHG/THG), Coherent anti-Stokes Raman Scattering and Stimulated Raman scattering (CARS/SRS), Fluorescence lifetime (FLIM) and epi-widefield fluorescence gains orthogonal data sets and addresses a broad range of requirements from different samples. Non-linear imaging techniques like TPEF or SHG/THG enable deep tissue penetration and the multi-channel approach allows multiplexing to distinguish between different imaging modalities that can be recorded simultaneously. Thus, a broad range of key uses in life science research including 3D imaging, label-free, deep tissue, in-vivo and intravital live-animal, whole organ, and whole slide imaging can be accommodated.

The MPX microscope: A turn-key and versatile multimodal multiphoton platform with a 360-frontend and fully integrated fs laser