CrestOptics SpA
Luca Clario, Francesco Bacchi
On Stand Workshop - Stand 8
Correlative multimodal bioimaging is the convergence point of technologies with different performances in terms of statistical sampling, number of simultaneously analyzed signals, temporal and spatial resolution. It combines the strengths of different imaging approaches to overcome the limitations of individual techniques and provides a more holistic understanding of the sample under investigation. Super resolution microscopy, such Structured Illumination Microscopy (SIM) and molecular localization-based approaches, provide a spatial resolution and a molecular localization precision able to explore the scale of macromolecular complexes in situ. However, its use is limited to restricted regions, and consequently few cells, and frequently no more than one or two parameters. Spinning Disk Confocal is a high-speed, high-sensitivity method to reject out-of-focus light in thick and large specimens: this makes it a very common choice for studying 3D structure of tissue sections, fast dynamic processes, and long-term time-lapse of live samples. This workshop shows a correlative multimodal microscopy approach that exploits the major advantages of Spinning Disk Confocal and SIM technologies to get the most out of a whole set of biological samples from different spatial scales. Data is collected separately using each modality, and the integration between the datasets is established post-acquisition through computational or analytical techniques to enable a more holistic understanding of the sample. Possible implementation of such techniques into an automated acquisition and analysis pipeline is discussed.